February 2017
Codif works with DNA GENSEE for authentification, security and traceability of its raw materials.
Recent developments in genetics have led to the establishment of powerful DNA-based method allowing plant species identification. Based on DNA sequences and the genetic signature of each plant, it was possible to evidence differences between various origins of species. This important development permitted to propose identification of plant species that were used to make plant extracts for cosmetics.
DNA barcoding provides a powerful tool for botanical identification but also for algae (CYanobacteria, Dinoflagellates, Diatoms, Brown algae, Euglenids, Green algae, Red algae) and the results were presented at the last edition of COSM’ING in Saint Malo last June. This can be used on raw materials for the perfumery, cosmetics and nutraceutics industries for authentication, security and traceability of the selected plants. Here we demonstrated that DNA barcoding is also able to discriminate between several geographical origins of one species.
Codif laboratories (producer of vegetal active ingredients for cosmetics) and DNA Gensee laboratories (biotechnology) , conducted analysis on a halophytic plant : Crithmum maritimum (Apiaceae), also known as Rock Samphire.
We have shown that four geographic accessions of Crithmum maritimum could be easily discriminated, based on their genetic sequences. On the four genetic markers tested, two were perfectly identical between all of the four samphire geographic origins tested.
Here are presented the two electrophoresis gels (Figure A and figure B) corresponding to the two markers that showes differences between the different origins.
The gel (Figures 1A) shows polymorphisms that allow to discriminate plants from Vendée from the other three origins . The gel (Figures 1B) also shows polymorphisms and can discriminate between [Vendée/ Brittany], Morocco and Brittany cultivated plants.
